Simple coating of zinc powder on foam tape based on a downscaling procedure for nitrate determination in vegetable samples


  • Narong Kotchabhakdi Department of Chemistry, Faculty of Sciences, Lampang Rajabhat Lampang University, Lampang 52100, Thailand


downscaling, Griess reaction, nitrate determination, nitrate reduction, zinc powder


Zinc-Coated Foam Tape (ZCFT) was combined with the Griess reagent for nitrate determination.  A portable chip of zinc powder coating was applied to double-sided PU foam tape with side dimensions of 1x1 cm and approximately 7.7 ± 0.4 mg of zinc.  The linear range of the proposed method was 2-10 mg L-1, and the obtained percent of nitrate reduction was 23-25%.  The real sample application was studied in the determination of nitrate in vegetable samples.  The results showed good agreement with the reference method (the NitraVer®5 nitrate reagent powder pillows).  Simple detection can be set up using a digital microscope with the ImageJ program for image processing and evaluation.  The proposed method, which offers the advantages of simple operation and low cost, can be used as rapid test kit to monitor the nitrate residue in vegetables. 


Campbell, W. H. (1999). Nitrate reductase structure, function and regulation: bridging the gap between biochemistry and physiology. Annual review of plant biology, 50(1), 277-303. DOI: 10.1146/annurev.arplant.50.1.277

Choodum, A., Boonsamran, P., NicDaeid, N., & Wongniramaikul, W. (2015). On-site semi-quantitative analysis for ammonium nitrate detection using digital image colourimetry. Science & Justice, 55(6), 437-445. DOI: org/10.1016/j.scijus.2015.05.001

Choodum, A., Tiengtum, J., Taweekarn, T., & Wongniramaikul, W. (2020). Convenient environmentally friendly on-site quantitative analysis of nitrite and nitrate in seawater based on polymeric test kits and smartphone application. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 243, 118812. DOI: org/10.1016/j.saa.2020.118812

Colla, G., Kim, H. J., Kyriacou, M. C., & Rouphael, Y. (2018). Nitrate in fruits and vegetables. Scientia Horticulturae, 237, 221-238. DOI: 10.1016/j.scienta.2018.04.016

Cortas, N. K., & Wakid, N. W. (1990). Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clinical chemistry, 36(8), 1440-1443. DOI: /10.1093/clinchem/36.8.1440

Eaton, A. D. (2005). Standard methods for the examination of water and wastewater: 21st ed. (2005). American Public Health Association, Washington DC.

Ellis, P. S., Shabani, A. M. H., Gentle, B. S., & McKelvie, I. D. (2011). Field measurement of nitrate in marine and estuarine waters with a flow analysis system utilizing on-line zinc reduction. Talanta, 84(1), 98-103. DOI: 10.1016/j.talanta.2010.12.028

FAO/WHO. (2013). Nitrate and potential endogenous formation of N-nitroso compounds (2013). WHO Food Additive series World Health Organization, Geneva.

Ferreira, F. T., Mesquita, R. B., & Rangel, A. O. (2020). Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva. Talanta, 219, 121183. DOI: 10.1016/j.talanta.2020.121183

Guo, D., Lou, C., Chen, M., Zhu, B., Zhang, P., Wang, N., & Zhu, Y. (2017). Determination of chloride and nitrate in dimethyl carbonate using a column-switching ion chromatography system. Analytical Methods, 9(19), 2840-2844. DOI: org/10.1039/C7AY00906B

Jaikang, P., Wangkarn, S., Paengnakorn, P., & Grudpan, K. (2019). Microliter operation for determination of nitrate-nitrogen via simple zinc reduction and color formation in a Well Plate with a Smartphone. Analytical Sciences, 35(4), 421-425. DOI: org/10.2116/analsci.18P497

Jayawardane, B. M., Wei, S., McKelvie, I. D., & Kolev, S. D. (2014). Microfluidic paper-based analytical device for the determination of nitrite and nitrate. Analytical chemistry, 86(15), 7274-7279. DOI:

Li, X., Zou, N., Wang, Z., Sun, Y., Li, H., Gao, C., ... & Wang, X. (2020). An electrochemical sensor for determination of nitrite based on Au nanoparticles decorated MoS 2 nanosheets. Chemical Papers, 74(2), 441-449. DOI: 10.1007/s11696-019-00885-9

Ma, L., Hu, L., Feng, X., & Wang, S. (2018). Nitrate and nitrite in health and disease. Aging and disease, 9(5), 938. DOI: 10.14336/AD.2017.1207

Ma, Z., Li, J., Hu, X., Cai, Z., & Dou, X. (2020). Ultrasensitive, Specific, and Rapid Fluorescence Turn‐On Nitrite Sensor Enabled by Precisely Modulated Fluorophore Binding. Advanced Science, 7(24), 2002991.

Martínková, E., Křžek, T., & Coufal, P. (2014). Determination of nitrites and nitrates in drinking water using capillary electrophoresis. Chemical papers, 68(8), 1008-1014. DOI: 10.2478/s11696-014-0548-4

Mir, S. A. (2007). An improved zinc reduction method for direct determination of nitrate in presence of nitrite. Asian Journal of Chemistry, 19(7), 5703.

Murray, E., Nesterenko, E. P., McCaul, M., Morrin, A., Diamond, D., & Moore, B. (2017). A colorimetric method for use within portable test kits for nitrate determination in various water matrices. Analytical Methods, 9(4), 680-687. DOI: org/10.1039/C6AY03190K

Pai, S. C., Su, Y. T., Lu, M. C., Chou, Y., & Ho, T. Y. (2021). Determination of Nitrate in Natural Waters by Vanadium Reduction and the Griess Assay: Reassessment and Optimization. ACS ES&T Water, 1(6), 1524-1532.

Patton, C. J., Fischer, A. E., Campbell, W. H., & Campbell, E. R. (2002). Corn Leaf Nitrate Reductase A Nontoxic Alternative to Cadmium for Photometric Nitrate Determinations in Water Samples by Air-Segmented Continuous-Flow Analysis. Environmental science & technology, 36(4), 729-735. DOI: org/10.1021/es011132a

Váradi, L., Breedon, M., Chen, F. F., Trinchi, A., Cole, I. S., & Wei, G. (2019). Evaluation of novel Griess-reagent candidates for nitrite sensing in aqueous media identified via molecular fingerprint searching. RSC advances, 9(7), 3994-4000. DOI: 10.1039/C8RA07656




How to Cite

Kotchabhakdi, N. . (2023). Simple coating of zinc powder on foam tape based on a downscaling procedure for nitrate determination in vegetable samples . Journal of Current Science and Technology, 12(1), 52–60. Retrieved from



Research Article