Practical applications of peripheral blood mononuclear cells (PBMCs) in immunotherapy preclinical research

Joanna Baran; Marta Rogalska; Lukasz Kuryk; Monika Staniszewska

Authors

Joanna Baran Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology (Laboratory of Pathogens Biology and Molecular Diagnostics): Poleczki 19, 02-822 Warsaw, Poland
Marta Rogalska Faculty of Chemistry, Warsaw University of Technology, (Laboratory of Pathogens Biology and Molecular Diagnostics): Poleczki 19, 02-822 Warsaw, Poland
Lukasz Kuryk National Institute of Public Health NIH - National Research Institute: Warsaw, Poland
Monika Staniszewska Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology (Laboratory of Pathogens Biology and Molecular Diagnostics): Poleczki 19, 02-822 Warsaw, Poland

Keywords:

cryopreservation, microfluidics, PBMC isolation, peripheral blood mononuclear cells (PBMCs), preclinical research, viruses

Abstract

In recent years, we have faced more and more new diseases caused by viruses and opportunistic infections. There are still no preclinical models that would improve the evaluation of the proposed therapies and the immune system's response to drugs and infections. That is why the models based on the peripheral blood mononuclear cells (PBMCs) have a great potential to evaluate the human body’s cellular, cytotoxic and humoral responses to vaccines and drugs. At the same time, studying these models may be the next stage of preclinical research, allowing for the initial verification of the tested factors before starting animal testing. This review has compiled the key information on the isolation, culture, cryopreservation, and application potential of PBMCs in the preclinical research focusing on viral pathogenesis and therapy.

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