Assessment of in vitro Anti-inflammatory and Anti-acne-inducing Bacterial Activities of Kameng (Eclipta prostrata Linn.) Cultivated in Suphan Buri Province, Thailand
DOI:
https://doi.org/10.59796/jcst.V14N2.2024.27Keywords:
Kameng extract, Anti-acne inducing bacterial activities, In vitro anti-inflammatory, Protein degradation, Proteinase inhibitory assaysAbstract
This research investigates extracts from Kameng (Eclipta prostrata Linn.), originally employed in traditional medicinal recipes, with the aim of developing an extract that can serve as an alternative to antibiotics. For this purpose, the anti-inflammatory and anti-acne-inducing bacterial activities of Kameng crude ethanol extract, grown in Suphan Buri Province were investigated, with anti-inflammatory properties assessed by the inhibition of protein degradation and proteinase inhibitory assays. Findings indicated the potential of Kameng extracts as anti-inflammatory drugs that reduce protein degradation and proteinase activities. Kameng extract concentrations ranging from 0.4 to 4.0 x 104 ppm inhibited protein degradation and proteinase activity by 14.66%-50.75% and 28.57%-72.08%, respectively. The agar disc diffusion method revealed that Kameng extracts had strong inhibitory effects against S. aureus and C. acnes, with inhibition zone diameters of 31.15±0.47 and 37.28±0.47 mm, respectively. The minimal inhibitory concentration (MIC) of Kameng extract against S. aureus and C. acnes was 5.0 mg/mL and 2.5 mg/mL while minimal bactericidal concentration (MBC) of Kameng extract against S. aureus and C. acnes was 20.0 mg/mL and 10.0 mg/mL, respectively. Results suggested the potential of Kameng extract to cure acne and skin inflammation as a herbal cosmetic product.
References
Adhikari, M., Thapa, R., Kunwar, R. M., Devkota, H. P., & Poudel, P. (2019). Ethnomedicinal uses of plant resources in the Machhapuchchhre rural municipality of Kaski District, Nepal. Medicines, 6(2), Article 69. https://doi.org/10.3390/medicines6020069
Ambu, G., Chaudhary, R. P., Mariotti, M., & Cornara, L. (2020). Traditional uses of medicinal plants by ethnic people in the Kavrepalanchok district, Central Nepal. Plants, 9(6), Article 759. https://doi.org/10.3390/plants9060759
Arunachalam, G., Subramanian, N., Pazhani, G. P., & Ravichandran, V. (2009). Anti-inflammatory activity of methanolic extract of Eclipta prostrata L.(Astearaceae). African journal of pharmacy and pharmacology, 3(3), 97-100.
Banjara, R. A., Jadhav, S. K., & Bhoite, S. A. (2012). Antibacterial activity of di-2-ethylaniline phosphate screened by paper disc diffusion method. Journal of Applied Pharmaceutical Science, 2(7), 230-233. https://doi.org/10.7324/JAPS.2012.2720
Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4_ts), 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
BGO Plant Database, The Botanical Garden Organization. (2019). Ecipta prostrate L. Retrieved 20 June 2019, from http://www.qsbg.org/Database/Botanic_Book%20full%20option/search_detail.asp? botanic_id=1583
Boonyaprapat, N., Chokchaicharernporn, O. (1999). Herbs and wood in Thai Traditional Medicine. The Prachachon Co., Ltd, Inc., Bangkok.
Buachard, S., Chanphuak, P., and Sratongwat, R. (2015). Antimicrobial Activity against Acne-Inducing Bacteria by Herbal Plant Extracts. Agricultural Science Journal, 46(3)(Suppl.), 317-320.
Cherdtrakulkiat, R., Boonpangrak, S., Pingaew, R., Prachayasittikul, S., Ruchirawat, S., & Prachayasittikul, V. (2015). Bioactive triterpenoids, antimicrobial, antioxidant and cytotoxic activities of Eclipta prostrata Linn. Journal of Applied Pharmaceutical Science, 5(3), 046-050. https://doi.org/10.7324/JAPS.2015.50308
Corvec, S., Dagnelie, M. A., Khammari, A., & Dréno, B. (2019). Taxonomy and phylogeny of Cutibacterium (formerly Propionibacterium) acnes in inflammatory skin diseases. In Annales de Dermatologie et de Vénéréologie, 146(1), 26-30. https://doi.org/10.1016/j.annder.2018.11.002
Department of Thai Traditional and Alternative Medicine Ministry of Public Health. (2019). Herbs in Thai Traditional Medicine Recipes with Cannabis. Retrieved 20 June, 2019, from: https://ockt.dtam.moph.go.th.
Gautam, T. P. (2011). Indigenous uses of some medicinal plants in Panchthar district, Nepal. Nepalese Journal of Biosciences, 1, 125-130. https://doi.org/10.3126/njbs.v1i0.7479
Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., & Rupasinghe, H. P. V. (2018). Influence of boiling, steaming and frying of selected leafy vegetables on the in vitro anti-inflammation associated biological activities. Plants, 7(1), Article 22. https://doi.org/10.3390/plants7010022
Jahan, R., Al-Nahain, A., Majumder, S., & Rahmatullah, M. (2014). Ethnopharmacological significance of Eclipta alba (L.) Hassk.(Asteraceae). International scholarly research notices, 2014, Article 385969. https://doi.org/10.1155/2014/385969
Lee, C. J., Chen, L. W., Chen, L. G., Chang, T. L., Huang, C. W., Huang, M. C., & Wang, C. C. (2013). Correlations of the components of tea tree oil with its antibacterial effects and skin irritation. Journal of Food and Drug Analysis, 21(2), 169-176. https://doi.org/10.1016/j.jfda.2013.05.007
Lertcanawanichakul, M., Tehsuan, K., & Sahoynpong, A. (2018). Anti-Acne Causing Bacteria Activity of Acne Gel Composed with Biocompounds Produced from Brevibacillus laterosporus. ASEAN Journal of Scientific and Technological Reports, 21(3), 1-7.
Pandey, M. K., Singh, G. N., Sharma, R. K., & Lata, S. (2011). Antibacterial activity of Eclipta alba (L.) Hassk. Journal of Applied Pharmaceutical Science, 1(7), 104-107.
Plant Genetic Conservation Project Under the Royal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn (RSPG). (2019). Eclipta prostrata (L.) L. Retrieved 20 June 2019. Fromhttp://www.rspg.or.th/plants_data/plantdat/asterace/eprost_1.htm.
Prachayasittikul, S., Wongsawatkul, O., Suksrichavalit, T., Ruchirawat, S., & Prachayasittikul, V. (2010). Bioactivity evaluation of Eclipta prostrata Linn. European Journal of Scientific Research, 44, 167-176.
Ramathibodi Poison Center. (2019). Toxicology on the general public; Antibiotics. Retrieved 22 June 2019,from https://med.mahidol.ac.th/poisoncenter/th/knowledge_general_population/29jun2016-1052-th
Raveesha, K. A., & Shrisha, D. L. (2013). Antidermatophytic activity of Eclipta prostrata L. against human infective Trichophyton and Microsporum spp. International Journal of Chemical and Analytical Science, 4(2), 136-138. https://doi.org/10.1016/j.ijcas.2013.05.003
Sangkaram, N., Sangthog, B., & Rattarm, R. (2021). Biological activities, total phenolic compound and TLC fingerprint of Eclipta prostrata (L.) L., Sphagneticola calendulacea (L.) Pruski and Sphagneticola trilobata (L.) Pruski. Journal of Science & Technology MSU, 40(4), 341-351.
Somkhow, P., Junlatat, J., Chalomphong, N., Prasert, T. and Mulateeka, P. (2022). The efficacy of acne gel containing Allium ascalonicum L. extracts. Journal of Traditional Thai Medical Research, 8(2), 99-110.
Supaphon, P. (2018). Antimicrobial, Antioxidant and Anti-alpha-glucosidase Activities of Crude Extracts from Fruits of Malpighia glabra. Thai Journal of Science and Technology, 28, 831-841.
Timalsina, D., & Devkota, H. P. (2021). Eclipta prostrata (L.) L.(Asteraceae): ethnomedicinal uses, chemical constituents, and biological activities. Biomolecules, 11(11), Article 1738. https://doi.org/10.3390/biom11111738
Zesch, A. (1988). Adverse reactions of externally applied drugs and inert substances. Dermatosen in Berufund Umwelt. Occupation and Environment, 36(4), 128-133.
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