The antibacterial activity and silk dyeing of the crude pigment extract from  J4 actinobacteria strain

Sittichai Urtgam; Tawatchai Sumpradit; Naruemol Thurnkul

doi:10.59796/jcst.V13N2.2023.262

Authors

Sittichai Urtgam Biology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University & Center of Excellence for Biodiversity, Naresuan University
Tawatchai Sumpradit Department of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University
Naruemol Thurnkul Microbiology Program, Faculty of Science and Technology, Pibulsongkram Rajabhat University

DOI:

https://doi.org/10.59796/jcst.V13N2.2023.262

Keywords:

Antibacterial activity, Silk dyeing, Actinobacterial pigment

Abstract

Actinobacteria represent a vast variety of prokaryotic microbes that produce a diverse group of secondary metabolites used in medical, agricultural, and industrial applications. The objectives in this study were to investigate the antibacterial activities and potential for silk dyeing of the crude extract from the actinobacterial strain J4. Initially, the antibacterial activities of the crude pigment extracts obtained from the actinobacterial strain J4 isolated from the wasp’s nest were determined. To extract the water-insoluble pigments used for antibacterial activity testing via the agar well diffusion method, ethyl acetate was applied during the pigment extraction. These extracts showed an inhibitory effect against Salmonella Typhimurium TISTR1472, Bacillus subtilis PSRU-01, and Enterococcus faecalis TISTR459. Secondly, evaluation of silk dyeing was carried out. We used the extractant as 70 % (v/v) ethanol in the alternative extraction process when the strain J4 was cultivated by solid-state fermentation with broken-milled rice to prepare actinobacterial dye for silk dyeing. The pigment extract obtained was tested for color stability based on washing methods, and the sensitivity to light was determined according to ISO105-C06:2010(E) and ISO 105-B02:2014(E), respectively. The color stability and sensitivity of the pigment extract to washing showed scores between 3-5, and the sensitivity to light demonstrated scores between 3-4. Taxonomically, the strain J4 was dark-violet colonies, and its water-soluble pigment diffused on sodium caseinate agar (SCA). To identify the strain J4, the phylogenetic identification based on partial sequencing of 16S rDNA was applied. The identification result indicated that the strain J4 was closely related to Streptomyces coacervatus (99.8% sequence similarity). In future, antibacterial activities of the crude extracts obtained from actinobacteria strain J4 should be tested with other food-borne pathogens. The development of Streptomyces dyes should also focus on the stability of the dyes during the silk dyeing process.

References

Abraham, J., & Chauhan, R. (2018). Profiling of red pigment produced by Streptomyces sp. JAR6 and its bioactivity. Biotech, 8(1), 1-9. https://doi.org/10.1007/s13205-017-1044-7

Al_husnan, L. A., & Alkahtani, M. D. (2016). Molecular identification of Streptomyces producing antibiotics and their antimicrobial activities. Annals of Agricultural Sciences, 61(2), 251-255. https://doi.org/10.1016/j.aoas.2016.06.002

Amal, A. M., Abeer, K. A., Samia, H. M., Nadia, A. E. N. H., KA, A., & HM, E. H. (2011). Selection of Pigment (melanin) production in Streptomyces and their application in printing and dyeing of wool fabrics. Research Journal of Chemical Sciences, 1(5), 22-28.

Ayed, A., Essid, R., Jallouli, S., Ben Zaied, A., Ben Fdhila, S., Limam, F., & Tabbene, O. (2022). Antifungal activity of volatile organic compounds (VOCs) produced by Streptomyces olivochromogenes S103 against Candida albicans. Euro-Mediterranean Journal for Environmental Integration, 7(2), 251–255. https://doi.org/10.1007/s41207-022-00302-w

Balachandran, C., Duraipandiyan, V., Arun, Y., Sangeetha, B., Emi, N., Al-Dhabi, N. A., ... & Perumal, P. T. (2016). Isolation and characterization of 2-hydroxy-9, 10-anthraquinone from Streptomyces olivochromogenes (ERINLG-261) with antimicrobial and antiproliferative properties. Revista Brasileira de Farmacognosia, 26(3), 285-295. https://doi.org/10.1016/j.bjp.2015.12.003

Chakraborty, I., Redkar, P., Munjal, M., Kumar, S. S., & Rao, K. B. (2015). Isolation and characterization of pigment producing marine actinobacteria from mangrove soil and applications of bio-pigments. Der Pharm. Lett, 7(4), 93-100.

Chen, W., Ye, K., Zhu, X., Zhang, H., Si, R., Chen, J., ... & Han, B. (2021). Actinomycin X2, an antimicrobial depsipeptide from marine-derived Streptomyces cyaneofuscatus applied as a good natural dye for silk fabric. Marine Drugs, 20(1), Article 16. https://doi.org/10.3390/md20010016

Conlon, J. M., Kolodziejek, J., & Nowotny, N. (2004). Antimicrobial peptides from ranid frogs: taxonomic and phylogenetic markers and a potential source of new therapeutic agents. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1696(1), 1-14. https://doi.org/10.1016/j.bbapap.2003.09.004

Dohra, H., Miyake, Y., & Kodani, S. (2017). Draft genome sequence of Streptomyces olivochromogenes NBRC 3561, a bioactive peptide-producing actinobacterium. Genome announcements, 5(39), e01048-17. https://doi.org/10.1128/genomea.01048-17

El-Naggar, N. E. A., & El-Ewasy, S. M. (2017). Bioproduction, characterization, anticancer and antioxidant activities of extracellular melanin pigment produced by newly isolated microbial cell factories Streptomyces glaucescens NEAE-H. Scientific reports, 7(1), 1-19. https://doi.org/10.1038/srep42129

Goodfellow, M., Kämpfer, P., Busse, H.J., Trujillo, M.E., Suzuki, K., Ludwig, W. and Whitman, W.B. (2012). Bergey’s Manual of Systematic Bacteriology. 2nd Ed. Vol 5. The Actinobacteria, Part A and B. New York: Springer-Verlag. ISBN 978-0-387-68233-4.

Gu, L., Zhang, K., Zhang, N., Li, X., & Liu, Z. (2020). Control of the rubber anthracnose fungus Colletotrichum gloeosporioides using culture filtrate extract from Streptomyces deccanensis QY-3. Antonie van Leeuwenhoek, 113(11), 1573-1585. https://doi.org/10.1007/s10482-020-01465-8

Janković, V., Marković, D., Nikodinovic-Runic, J., Radetić, M., & Ilic-Tomic, T. (2023). Eco-friendly dyeing of polyamide and polyamide-elastane knits with living bacterial cultures of two Streptomyces sp. strains. World Journal of Microbiology and Biotechnology, 39(1), 1-14. https://doi.org/10.1007/s11274-022-03473-4

Jose, P. A., & Jebakumar, S. R. D. (2013). Non-streptomycete actinomycetes nourish the current microbial antibiotic drug discovery. Frontiers in microbiology, 4, Article 240. https://doi.org/10.3389/fmicb.2013.00240

Karuppiah, V., Aarthi, C., Sivakumar, K., & Kannan, L. (2013). Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4. Asian Pacific journal of tropical biomedicine, 3(8), 650-656. https://doi.org/10.1016/S2221-1691(13)60131-8

Kermeoglu, F., Aksoy, U., Kalender, A., Oztan, M. D., Oguz, E. I., & Kıyan, M. (2018). Determination of the minimum inhibitory concentrations of alexidine and chlorhexidine against Enterococcus faecalis and Candida albicans: An in vitro study. Cureus, 10(2), Article e2221. https://doi.org/10.7759/cureus.2221

Kramar, A, Kostic M. M. (2022) Bacterial secondary metabolites as biopigments for textile dyeing. Textiles, 2(2), 252–264. https://doi.org/10.3390/textiles2020013

Kramar, A., Ilic-Tomic, T., Petkovic, M., Radulovic, N., Kostic, M., Jocic, D. & Nikodinovic-Runic, J. (2014). Crude bacterial extracts of two new Streptomyces sp. isolates as bio-colorants for textile dyeing. World Journal Microbiology Biotechnology, 30, 2231–2240.

Kulkarni, V. M., Gangawane, P. D., Patwardhan, A. V., & Adivarekar, R. V. (2014). Dyeing of silk/ wool using crude pigment extract from an isolate Kocuria flava sp. HO-9041. Octa Journal of Environmental Research, 2(4), 314-320.

Li, X., Li, E., Zhu, Y., Teng, C., Sun, B., Song, H., & Yang, R. (2012). A typical endo-xylanase from Streptomyces rameus L2001 and its unique characteristics in xylooligosaccharide production. Carbohydrate research, 359, 30-36. https://doi.org/10.1016/j.carres.2012.05.005

Locci, R. (1989). Streptomycetes and related genera. In: Williams ST, Sharpe ME, Holt JG (Eds) Bergey’s manual of systematic bacteriology. London, England: Williams and Wilkins.

Maiden, N. A., Noran, A. S., Fauzi, M. A. F. A., & Atan, S. (2017). Screening and characterisation of chitinolytic microorganisms with potential to control white root disease of Hevea brasiliensis. Journal of Rubber Research, 20(3), 182-202. https://doi.org/10.1007/BF03449151

Manikkam, R., Venugopal, G., Ramasamy, B., & Kumar, V. (2015). Effect of critical medium components and culture conditions on antitubercular pigment production from novel Streptomyces sp D25 isolated from Thar desert, Rajasthan. Journal of Applied Pharmaceutical Science, 5(06), 015-019. https://doi.org/10.7324/JAPS.2015.50603

Meena, L. I., Rajeswari, E., Ahiladevi, P., Kamalakannan, A., & Kalaiselvi, T. (2022). Antifungal potential of Streptomyces rameus GgS 48 against mungbean root rot [Rhizoctonia bataticola (Taub.) Butler]. Journal of Biosciences, 47, 10. https://doi.org/10.1007/s12038-021-00244-5

Meng-Xi, L. I., Hui-Bin, H., Jie-Yun, L., Jing-Xiao, C. A. O., & Zhen-Wang, Z. (2021). Antibacterial performance of a Streptomyces spectabilis strain producing metacycloprodigiosin. Current Microbiology, 78, 2569–2576. https://doi.org/10.1007/s00284-021-02513-w

Nair, A. S., Kumar, B. P., & Geo, J. A. (2017). Microbial production of textile grade pigments. African Journal of Microbiology Research, 11(42), 1532-1537. https://doi.org/10.5897/AJMR2017.8205

Phankhajon, K., Somdee, A., & Somdee, T. (2016). Algicidal activity of an actinomycete strain, Streptomyces rameus, against Microcystis aeruginosa. Water Science and Technology, 74(6), 1398-1408. https://doi.org/10.2166/wst.2016.305

Praveen, V., & Tripathi, C. K. M. (2009). Studies on the production of actinomycin‐D by Streptomyces griseoruber–a novel source. Letters in applied microbiology, 49(4), 450-455. https://doi.org/10.1111/j.1472-765X.2009.02689.x

Ramesh, C., Vinithkumar, N. V., Kirubagaran, R., Venil, C. K., & Dufossé, L. (2020). Applications of prodigiosin extracted from marine red pigmented bacteria Zooshikella sp. and actinomycete Streptomyces sp. Microorganisms, 8(4), Article 556. https://doi.org/10.3390/microorganisms8040556

Radhakrishnan, M., Gopikrishnan, V., Vijayalakshmi, G., & Kumar, V. (2016). In vitro antioxidant activity and antimicrobial activity against biofilm forming bacteria by the pigment from Desert soil Streptomyces sp D25. Journal of Applied Pharmaceutical Science, 6(6), 148-150. https://doi.org/10.7324/JAPS.2016.60626

Shibazaki, A., Omoto, Y., Kudo, T., Yaguchi, T., Saito, A., Ando, Mikami Y. & Gonoi, T. (2011). Streptomyces coacervatus sp. nov., isolated from the intestinal tract of Armadillidium vulgare. International journal of systematic and evolutionary microbiology, 61(5), 1073-1077. https://doi.org/10.1099/ijs.0.019091-0

Smaoui, S., Mathieu, F., Elleuch, L., Coppel, Y., Merlina, G., Karray-Rebai, I., & Mellouli, L. (2012). Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain. World Journal of Microbiology and Biotechnology, 28, 793-804. https://doi.org/10.1007/s11274-011-0872-6

Stankovic, N., Radulovic, V., Petkovic, M., Vuckovic, I., Jadranin, M., Vasiljevic, B., & Nikodinovic-Runic, J. (2012). Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. Applied microbiology and biotechnology, 96(5), 1217-1231. https://doi.org/10.1007/s00253-012-4237-3

Suomi, P. (2021). All Fiber Arts. Fungi dye recipe. Pisolithus arhizus - Herne Kuukunen (FI). Retrieved July 24, 2021. From the https://www.allfiberarts.com/2017/fungi-dye-pisolithus-arhizus.htm.

Urtgam, S. and Thurnkul, N. (2021). Application of the pigment of actinobacteria isolated from the wasps nest soil for dyeing silk fibers. Life Sciences and Environment Journal, 22 (2), 166-177. https://doi.org/10.14456/lsej.2021.4

Velmurugan, P., Kim, M. J., Park, J. S., Karthikeyan, K., Lakshmanaperumalsamy, P., Lee, K. J., ... & Oh, B. T. (2010). Dyeing of cotton yarn with five water soluble fungal pigments obtained from five fungi. Fibers and Polymers, 11, 598-605. https://doi.org/10.1007/s12221-010-0598-5

Vijayabharathi, R., Bruheim, P., Andreassen, T., Raja, D. S., Devi, P. B., Sathyabama, S., & Priyadarisini, V. B. (2011). Assessment of resistomycin, as an anticancer compound isolated and characterized from Streptomyces aurantiacus AAA5. The Journal of Microbiology, 49(6), 920-926. https://doi.org/10.1007/s12275-011-1260-5

Wang, J., Huang, Y., Lin, S., Liu, F., Song, Q., Peng, Y., & Zhao, L. (2012). A strain of Streptomyces griseoruber isolated from rhizospheric soil of Chinese cabbage as antagonist to Plasmodiophora brassicae. Annals of microbiology, 62(1), 247-253. https://doi.org/10.1007/s13213-011-0253-2

Zhang, H., Zhan, J., Su, K., & Zhang, Y. (2006). A kind of potential food additive produced by Streptomyces coelicolor: characteristics of blue pigment and identification of a novel compound, λ-actinorhodin. Food Chemistry, 95(2), 186-192. https://doi.org/10.1016/j.foodchem.2004.12.02