Clinicopathological significance of FANCA mRNA expression in Thai patients with breast cancer

Pensri Saelee; Tanett Pongtheerat; Thanet Sophonnithiprasert; Worapoj Jinda

Authors

Pensri Saelee Division of Research and Academic Support, National Cancer Institute, Bangkok 10400, Thailand
Tanett Pongtheerat Unit of Biochemistry, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
Thanet Sophonnithiprasert Unit of Biochemistry, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
Worapoj Jinda Division of Research and Academic Support, National Cancer Institute, Bangkok 10400, Thailand

Keywords:

breast cancer, clinicopathological significance, FANCA mRNA expression, tumor suppressor gene

Abstract

The Fanconi anemia complementation group A (FANCA) gene is a potential tumor suppressor gene due to the repair of DNA damage mechanisms, and it remains a candidate as a cancer predisposition gene of breast cancer. In this study, the altered FANCA mRNA expression and the association with their clinicopathological data were investigated in patients with breast cancer. A total of 79 breast tumors from patients who did not receive chemotherapy or radiotherapy and their corresponding normal breast tissues were determined for the FANCA mRNA expression level using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The association between FANCA mRNA expression and patient clinicopathological features were analyzed using Chi-square tests and survival analysis was examined by the Kaplan-Meier method and Cox regression analysis. The FANCA mRNA expression level was overexpressed and underexpressed in breast cancers, with the frequency of 20 out of 72 (27.8%) and 7 out of 59 (11.9%), respectively. The FANCA over-expression was found associated with triple-negative breast cancer patients (P=0.048), Odds ratio; 95%CI (3.15; 0.98-10.09). Additionally, FANCA under-expression has been observed with low tumor grade I (P=0.035). This study concluded that the over-expression of FANCA mRNA expression used for screening hormone receptor status potentially was associated with triple negative-breast cancer while under-expression used for tumor grading biomarkers potentially in breast cancer patients was associated with low tumor grade.

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