The efficacy of povidone-iodine and normal saline on excision wound in mice


  • Supparit Pakdeethai Pathobiology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
  • Watchara Chongsa Physiology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
  • Tadsanee Punjanon Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
  • Suwichak Chaisit Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
  • Cherdsak Boonyong Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand
  • Pataweekorn Ketkomol Pharmacology and Toxicology Unit, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand


collagen production, excision wound, epidermis, histoarchitecture, mice, povidone-iodine, wound healing


In cleaning wounds, little attention has been given to the solutions, waiting for the natural process without the user agent, but these problems can cause chronic injuries and inflammation. Povidone-iodine is a tropical antiseptic agent that popularly used to prevent infection and aid healing of wounds. However, the effects of povidone-iodine on the histologic change of excision wound healing have not been reported.  Therefore, the objective was to assess the histological influences of 10% povidone-iodine ointment on healing process of experiment excision wounds in mice. Albino ICR mice were randomly divided into two groups with six mice each; a normal saline-treated group and a povidone-iodine-treated group. Wounds were created in all mice and treated with normal saline or povidone-iodine once daily for 14 days. The wound areas were measured on days 2, 5, 8, and 14. Then, a percentage of wound contraction was calculated. The histoarchitecture of the wound was observed on days 14 by staining with hematoxylin-eosin and Masson’s trichrome. The results showed that the average percentage of wound contraction of the povidone-iodine-treated group was significantly higher than the normal saline-treated group on days 5, 8, and 14. The histoarchitecture studies of the povidone-iodine-treated group showed a thickening of the epidermis and dense deposition of collagen fiber when compared to the normal saline-treated group. These findings indicated that povidone-iodine treatment could accelerate the wound healing processes.


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How to Cite

Pakdeethai, S., Chongsa, W. ., Punjanon, T., Chaisit, S., Boonyong, C., & Ketkomol, P. (2023). The efficacy of povidone-iodine and normal saline on excision wound in mice. Journal of Current Science and Technology, 13(1), 83–90. Retrieved from



Research Article