Comparison of bioactive compounds contained in discard solid state culture and Cordyceps militaris fruiting body wines


  • Sasirindara Labua Faculty of Food Technology, College of Agricultural Innovation and Food Technology, Rangsit University, Pathum Thani 12000, Thailand
  • Tanatya Kenkhunthot Faculty of Agricultural Innovation, College of Agricultural Innovation and Food Technology, Rangsit University, Pathum Thani 12000, Thailand


antioxidant activity, bioactive compound, Cordyceps millitaris fruiting body wine, discard solid state culture wine


For centuries, bioactive compounds such as cordycepin and phenolic compounds, found in fruiting bodies and solid-state cultures of Cordyceps millitaris, have shown antioxidant activities which promote positive immune modulating effects.  Wine is one of the most popular alcoholic beverages consumed worldwide which contains bioactive compounds and phytochemical components such as resveratrol, flavonoids, polysaccharides and phenolic compounds.  Daily moderate wine consumption promotes longevity and prevents disease.  Based on this, the aim of this research is to compare the bioactive compounds and antioxidant activity in discard solid state culture and C. millitaris fruiting body wines which benefits health and promotes positive immune modulating effects.  Each wine was collected to assess alcohol content, total phenolic compounds, antioxidant activity and cordycepin content.  In this study, alcohol content, antioxidant activity by DPPH (1, 1diphenyl-2-picryl hydrazyl) radical scavenging method and total phenolic compounds in discard solid state culture and C. millitaris fruiting body wines increased during fermentation compared to initial amounts.  The maximum ethanol content of both wines was quite similar which was about 11.20-11.70% (v/v).  The total phenolic compounds corresponding to the absorbance of gallic acid and antioxidant activity of discard solid culture medium wine was significantly higher than that of C. millitaris fruiting body wine.  The highest measured antioxidant activity and total phenolic compounds in discard solid state culture wine for 30 days were 118.18 ± 2.82 mg AAE/L and 489.88 ± 8.52 mg GAE/L, respectively.  Cordycepin, a nucleic acid antibiotic found in discard solid state culture and C. millitaris fruiting body wines fermented for 30 days was 7.96 ± 0.45 mg/kg and 66.78 ± 0.08 mg/kg, respectively.  This study concludes that bioactive compounds and antioxidant activity in discard solid state culture wine were significantly higher which may benefit health in preventing damages and encourage disease prevention.


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How to Cite

Labua, S., & Kenkhunthot, T. (2023). Comparison of bioactive compounds contained in discard solid state culture and Cordyceps militaris fruiting body wines. Journal of Current Science and Technology, 13(1), 74–82. Retrieved from



Research Article