Pseudomonas aeruginosa SWUC02 Cell-Free Culture as a Potential Antimicrobial Agent Against Household Antibiotics-Resistant Staphylococcus aureus

Kotchanat  Srisangchun; Angkana  Yoopom; Rasiyakapat  Boonyangchanichkul; Prawat  Aungpraphapornchai; Kwannan  Nantavisai; Onanong Pringsulaka; Siriruk  Sarawaneeyaruk

doi:10.59796/jcst.V13N3.2023.1349

Authors

Kotchanat Srisangchun Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Angkana Yoopom Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Rasiyakapat Boonyangchanichkul Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Prawat Aungpraphapornchai Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Kwannan Nantavisai Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, 10110, Thailand
Onanong Pringsulaka Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Siriruk Sarawaneeyaruk Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand

DOI:

https://doi.org/10.59796/jcst.V13N3.2023.1349

Keywords:

MRSA, S. aureus, antagonists, phenazine, Pseudomonas

Abstract

Contamination of household environments with pathogenic bacteria poses a significant risk of foodborne illnesses. This study aimed to investigate the effectiveness of cell-free culture obtained from Pseudomonas aeruginosa SWUC02 (CF-SWUC02) against Staphylococcus aureus, a common pathogen associated with food poisoning outbreaks. The antagonistic activity of P. aeruginosa SWUC02 and CF-SWUC02 against various pathogenic bacteria, particularly S. aureus, was assessed. The active antimicrobial compounds produced by P. aeruginosa SWUC02 demonstrated resistance to protease enzymes and high temperatures. Optimal culture conditions for inhibiting S. aureus were determined as LB media supplemented with 0.01% CuCl₂, inoculated with 1x10⁵ CFU.mL^-1 of P. aeruginosa SWUC02, and incubated at 32°C with agitation (100 rpm) for 12 days. Eighty-six S. aureus isolates were collected from household environments and tested for antibiotic resistance, with 55 isolates exhibiting resistance to penicillin, and 17 isolates were identified as methicillin-resistant S. aureus (MRSA). CF-SWUC02 demonstrated inhibitory effects against all S. aureus isolates, including MRSA. In conclusion, CF-SWUC02 displayed antagonistic activity against drug resistant S. aureus, suggesting its potential as a valuable resource for combating these pathogens. Furthermore, the presence of heat and protease stable antimicrobial compounds in CF-SWUC02 highlights the need for further investigation to explore their potential applications in the field of antimicrobial research.

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