In vitro Survival of Microencapsulated Canine-Specific Probiotics Under Simulated Gastrointestinal Tract Conditions and During Storage

Ngamlak  Foongsawat; Sirinthorn Sunthornthummas; Achariya Rangsiruji; Siriruk Sarawaneeyaruk; Kedvadee Insian; Onanong Pringsulaka

doi:10.59796/jcst.V13N3.2023.1121

Authors

Ngamlak Foongsawat Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Sirinthorn Sunthornthummas National Biobank of Thailand (NBT), National Science and Technology Development Agency, Pathum Thani, Thailand 12120
Achariya Rangsiruji Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Siriruk Sarawaneeyaruk Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Kedvadee Insian Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand
Onanong Pringsulaka Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand

DOI:

https://doi.org/10.59796/jcst.V13N3.2023.1121

Keywords:

Enterococcus hirae, Ligilactobacillus animalis, probiotic, dogs, microencapsulation, goat milk

Abstract

The survival of probiotics in the gastrointestinal system of dogs is crucial for them to provide health benefits. However, probiotics must also endure various physical conditions during commercial production and storage. Therefore, this study employed the microencapsulation technique to ensure the survival of probiotics using alginate as the encapsulation material, both alone and in combination with goat milk (alginate-goat milk). The study assessed the survival rates of two probiotic LAB strains, Enterococcus hirae Pom 4 and Ligilactobacillus animalis FB2, in both types of matrices under simulated dog gastrointestinal conditions, during food production, and 28 days of refrigeration at 4°C. The findings revealed that alginate-goat milk microcapsules had the highest encapsulation yield, and the viability of microencapsulated LAB cells in the alginate-goat milk matrix was the best protection for both probiotic strains under all conditions, including pasteurization temperature. Even after pasteurization, viable counts exceeding 6 log cfu/g were observed, indicating the promising application of alginate-goat milk microcapsules for optimal protection, enabling probiotics to survive until they reach the intended site and provide health benefits to dogs.

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