Extraction, Characterization and Antioxidant Capacity of Biopolymers from Nostoc commune using Hot Water and Combined Hot Water and Ultrasound-Assisted Extraction

Boonyakorn Boonyathan; Natta Laohakunjit; Apiradee Uthairatanakij; Nattapon Kaisangsri

Authors

Boonyakorn Boonyathan Department of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Natta Laohakunjit Department of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Apiradee Uthairatanakij Department of Postharvest Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Nattapon Kaisangsri Agricultural and Functional Foods Processing Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Keywords:

Antioxidant Activity, Pseudoplastic Fluids, Biopolymers, Nostoc commune, Extraction Methods

Abstract

Nostoc commune Vaucher, commonly known as “HedLarb,” is a species of cyanobacteria (blue-green algae), composing of various bioactive compounds, especially biopolymers (polysaccharides and proteins). The aim of this research was to study the best extraction conditions, in terms of physicochemical properties, molecular weight and rheological behavior, of biopolymers from N. commune; hot water extraction and combined hot-water and ultrasound-assisted extraction (UAE) were tested. The best condition for hot-water extraction was four times of extraction, at a ratio of solid to liquid of 1:60 (w/v) and temperature of 90 °C for 3 h. On the other hand, the best condition for combined hot-water and ultrasound-assisted extraction was four times of extraction, at a ratio of solid to liquid of 1:60 g/mL, ultrasonic power of 400 W at 80 °C for 30 min. The biopolymers from the best conditions of both hot water and combined hot-water and ultrasound-assisted extraction exhibited Mw of 4.49x10⁶ and 2.32x10⁶ Da, respectively. The extracted biopolymers via hot-water extraction and combined hot-water and ultrasound-assisted extraction showed ABTS^·+ antioxidant capacity of 92.03% and 95.35%, and DPPH^· antioxidant capacity of 39.13% and 42%, respectively. Rheological behaviors of the extracted biopolymers were pseudo-plastic, with weak gel property at a concentration of 0.8 % (w/v).

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